Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITKTLPLTDIHRHLDGNIRIQTILELGQQYNLDLPAYDIESLRPHVQVMDNQPDLLSFLSKLDWGVKVLASL-DACKRIAFENMQDAAQQGLDYVELRFSPGYMGMAHQLPL-----------EGVVEAVIAGVKEGSQAYGVKANLIGIMSRTFGQEACEKELNALLAHKND--IKALDLAGDELGFPGNLFIDH---FKKARDAGWHITVHAGEAAGPESIWQAIEELGAERIGHGVKAVQDLKLLDYLAKHQIGIESCLTSNIQTNTVPSLAEHPLKTFLEHGVLATINTDDPAVEGIEIQHEYLTAAPLAGLSPEQIYTAQENGLKIAFLSEQEKDELRQKYQ 1FKX Chain:A ((7-332)) ------PKVELHVHLDGAIKPETILYFGKKRGIALPADTVEELR-NIIGMDKPLSLPGFLAKFDYYMPVIAGCREAIKRIAYEFVEMKAKEGVVYVEVRYSPHLLANSKVDPMPWNQTEGDVTPDDVVDLVNQGLQEGEQAFGIKVRSILCCMRH--QPSWSLEVLELCKKYNQKTVVAMDLAGDETIEGSSLFPGHVEAYEGAVKNGIHRTVHAGEVGSPEVVREAVDILKTERVGHGYHTIEDEALYNRLLKENMHFEVCPWSSYLTGAWDPKTTHAVVRFKNDKANYSLNTDAPLIFKSTLDTDYQMTKKDMGFTEEEFKRLNINAAKSSFL-------------

General information: TITO was launched using: RESULT: Template: 1FKX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1802 29240 16.23 94.63 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : 16.23 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.371

(partial model without unconserved sides chains): PDB file : Tito_1FKX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FKX-query.scw PDB file : Tito_Scwrl_1FKX.pdb: