Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYLRPVMNSSNSHFNEVDPTLINETIPLFEKVPFNPIYPSKFKFIDLFAGIGGFRLAFQNLGGRCVFSSEIDLAAQKTYTANFGDTPYGDITLESTKDAIPDNFDILCGGFPCQAFSIAGKRSGFEDTRGTLFFDVADIIKRKQPKAFFLENVKGLVSHDKGKTLRTILQVLRE-DLGYYVPEPKIINAKDFGVPQNRERIFIVGFHKDTGVHDFEYPTPTG-----KKVCFADVKEENVVPTK--YYLSNQYL-QTLERHKAHHESKGNGFGYAIIPDNSIANAIVVGGMGRERNLVIDHRITDYTPTTRIKGEVNRQGIRKMTPREWARLQGFPDNFKIIVSDAAAYKQFGNSVAVPAIQATANLILEKLKMK 8MHT Chain:A ((12-325)) -----------------------------------------LRFIDLFAGLGGFRLALESCGAECVYSNEWDKYAQEVYEMNFGEKPEGDIT-QVNEKTIPDH-DILCAGFPCQAFSISGKQKGFEDSRGTLFFDIARIVREKKPKVVFMENVKNFASHDNGNTLEVVKNTMNELDYSFHA---KVLNALDYGIPQKRERIYMICFRNDLNIQNFQFPKPFELNTFVKDLLLPDSEVEHLVIDRKDLVMTNQEIEQTTPKTVRLGIVGKGGQGERIYSTRGIAITLSAYGGG----------IFAKTGGYLVNGKT-----RKLHPRECARVMGYPDSYKVHPSTSQAYKQFGNSVVINVLQYIAYNIGSSLNFK

General information: TITO was launched using: RESULT: Template: 8MHT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1554 -58857 -37.87 -192.97 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -37.87 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.499

(partial model without unconserved sides chains): PDB file : Tito_8MHT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-8MHT-query.scw PDB file : Tito_Scwrl_8MHT.pdb: