Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYDVALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAV----------------------------HAVGGTYGEEVVLPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLIQLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQ---------ADLVIDATKGSIKGETGIQIMKPGGRYVALNDL--------PDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKGAFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK 5K1S Chain:A ((18-357)) MKAVVLRSFGEAGNLKMETMPMPRP-GRGEVLLRVHACGVCYHDVINRRGNLP--RT-SVPAILGHEAAGEVIEVGPDTPGWKTGDRAATLQRMSCGDCALCRSGRNSLCKTDNRFFGEELPGGYAQFMVAPVGGLGRVPASLPWNEAATVCCTTGTAVHTVRTRGKVRAGETVLITGASGGVGLSSVQLARLDGARVIAVTSS--EAKVQAL-KEAGADEVIVSRGLDFASDVRKRTQGAGVDVAVEIV-GSATFDQTLKSMAPGGRVVVVGNLESGMVQLNPGLVIVKELE-ILGAYATTQAELD-EA----LRLTATGGVRQFVTD--AVPLAEAAKAHFRLENREVAGRLVL----

General information: TITO was launched using: RESULT: Template: 5K1S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1689 6332 3.75 21.46 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : 3.75 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.518

(partial model without unconserved sides chains): PDB file : Tito_5K1S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5K1S-query.scw PDB file : Tito_Scwrl_5K1S.pdb: