Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLKGASPMKAVTWQGKQKMEIRNVD--DPKILEPTDAIIRITATAICGSDLHLYHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNI-SCGHCHFCLNHMESQC---DESNPD---QLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLSDILPTAYWSVEN-AGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKAANYNGVETYFLEDTAQAGTE-LYELAKGGADVIIDCVG-MDGLEPVKEKAKNLVSLQSGTISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPII------HLMPKIYAMIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP 1U3T Chain:A ((12-372)) ----------AVLWELKKPFSIEEVEVAPPKAHE---VRIKMVAVGICGTDDHVVS-GTMVTPLPVILGHEAAGIVESVGEGVTTVKPGDK-VIPLAIPQCGKCRICKNPESNYCLKNDVSNPQGTLQDGTSRFTCRRKPIHHFLGISTFSQYTVVDENAVAKIDAASPLEKVCLIGCGFSTGYGSAVNVAKVTPGSTCAVFGLGGVGLSAIMGCKAAGAARIIAVDINKDKFAKAKELGATECINPQDYKKPIQEVLKEMTDGGVDFSFEVIGRLDTM-----------------MASLLCCHEAC---GTSVIVGV---PPDSQNLSMNPMLLLTGRTWKGAILGGFKSKECVPKLVADFMAKKFSLDALITHVLPFEKINEGFDLL--HSGKSIRTIL--

General information: TITO was launched using: RESULT: Template: 1U3T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2164 -45137 -20.86 -131.59 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -20.86 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.368

(partial model without unconserved sides chains): PDB file : Tito_1U3T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1U3T-query.scw PDB file : Tito_Scwrl_1U3T.pdb: