Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKQIGVLQMRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQPNKEHMDMPSLHSLEHLTAELIRNHADY----IVDWSPMGCQTGFYLTVLNHESYEDILDVLEATMKDVL---EATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA 1JOE Chain:A ((9-149)) ------------------VDHTKMNAPAVRIAKTMLTPKGDNITVFDLRFCIPNKEILSPKGIHTLEHLFAGFMRDHLNGDSIEIIDISPMGCRTGFYMSLIGTPNEQKVSEAWLASMQDVLGVQDQASIPELNIYQCGSYTEHSLEDAHEIAKNVIAR---------

General information: TITO was launched using: RESULT: Template: 1JOE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 568 -18771 -33.05 -140.08 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -33.05 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.502

(partial model without unconserved sides chains): PDB file : Tito_1JOE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1JOE-query.scw PDB file : Tito_Scwrl_1JOE.pdb: