Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEEESMRIACYGVRPNEIDFFEKLNIYH-YDLSLYEELLTHDNIVSAKDHDAVLLRG-NCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPNAIAELSLTFAMMLLRNTAYTTIRTSFK-DFRVDEQMFSREIRNCTVGIIGTGRIGLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPKARQLR 2YQ4 Chain:A ((3-331)) ------KIAMYNVSPIEVPYIEDWAKKNDVEIKTTDQALTSATVDLAEGCSSVSLKPLGPVDEEVVYQKLSEYGVKCIGLRIVGFNTINFDWTKK--LLVTNVPVYSPRAIAEMTVTQAMYLLRKIGEFRYRMDHDHDFTWPSNLISNEIYNLTVGLIGVGHIGSAVAEIFSAMGAKVIAYDVAYNPEFEPFLTYTDFDTVLKEADIVSLHTPLFP-STENMIGEKQLKEMKKSAYLINCARGELVDTGALIKALQDGEIAGAGLDTLAGESSYFGHTGLTDSEIPE-DYKTLAKM-PNVVITPHSAFYTETSIRNMVQICLTDQLTIAKGGRPRSIV---------

General information: TITO was launched using: RESULT: Template: 2YQ4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1687 -46847 -27.77 -144.59 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -27.77 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.539

(partial model without unconserved sides chains): PDB file : Tito_2YQ4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YQ4-query.scw PDB file : Tito_Scwrl_2YQ4.pdb: