Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMSNSSVIDAMCYNNSVKSKMKWRKDMDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGVLPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDEVMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDEVTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDAL-TLEEVAQAV---KNGTIDD----CLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIALFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE 3HAX Chain:A ((47-269)) ----------------------------GVINLDKPPGPTSHEVVAWIKKILNLEKAGHGGTLDPKVSGVLPVALEKATRVVQALLPAGKEYVALMHL-------------------HGDVPEDKIIQVMKEFEGEIIQRPPLRSAVK-----------------RRLRTRKVY-YIEVLEIEGRDVL------FRVGVEAGTYIRSLIHHIGLALGVGAHMSELRRTRSGPFKEDETLITLHDLVDYYYFWKEDGIEEYFRKAIQPMEKAVEHLPKVWIKDSAVAAVTHGADL-----------------------------------------------

General information: TITO was launched using: RESULT: Template: 3HAX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1023 -83064 -81.20 -386.34 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -81.20 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.400

(partial model without unconserved sides chains): PDB file : Tito_3HAX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3HAX-query.scw PDB file : Tito_Scwrl_3HAX.pdb: