Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACVAGNSYGIGASVT-LPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFGPLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGR-TLAAEEDFVQAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFW-LNAPFYPTDRINGTGDSLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE 4C5N Chain:A ((5-267)) ------KVLTIAGSDTSAGAGMQADLKTFQELDTYGMVALTAIVTMDKDTWSHDVTPLPMDVFEKQLE-TALSIGPDAIKTGMLGTEEIIKRAGEVYEASNAQYFVVDPVM--------LNPGNTEAMIKYLLPKATVVTPNLFEAGQLSGLGKLNSIEDMKKAATIIFDKGAQHVIIKGGKALDQDKS----YDLYYDGQTFYQLTTDMFQQSYNHGAGCTFAAATTAYLANGKSPKEAVISAKAFVASAIKNGWKMNDFVGPVDHGAYNRIE

General information: TITO was launched using: RESULT: Template: 4C5N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1557 5032 3.23 19.97 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : 3.23 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.485

(partial model without unconserved sides chains): PDB file : Tito_4C5N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4C5N-query.scw PDB file : Tito_Scwrl_4C5N.pdb: