Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAPPSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRNIFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN 4UC2 Chain:A ((25-149)) ----------------------------KPDEWYDNLNKPWWNPPRWVFPLAWTSLYFLMSLAAMRVAQLEGSGQA---LAFYAAQLAFNTLWTPVFFGMKRMATALAVVMVMWLFVAATMWAFFQLDTWAGVLFVPYLIWATATTGLNFEAMRLN

General information: TITO was launched using: RESULT: Template: 4UC2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 452 -99255 -219.59 -794.04 target 2D structure prediction score : 0.81 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -219.59 2D Compatibility (Sec. Struct. Predict.) : 0.81 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.384

(partial model without unconserved sides chains): PDB file : Tito_4UC2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4UC2-query.scw PDB file : Tito_Scwrl_4UC2.pdb: