Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTAEKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQGGYTRILKTEPRRGDGAPMVVLELV 5X8T Chain:O ((19-126)) ----RLSRPADQRRALLRGLTTQLLKHGRIKTTRAKASAMRKYVDKMITLAKEGSLHKRRQALGFI----------YEKQIVHA----LFAEVPERYGDRNGGYTRIIRTLPRRGDNAPMAYIELV

General information: TITO was launched using: RESULT: Template: 5X8T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain O - contact count / total energy / energy per contact / energy per residue : 377 -42521 -112.79 -393.71 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain O : 0.85 3D Compatibility (PKB) : -112.79 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.721

(partial model without unconserved sides chains): PDB file : Tito_5X8T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5X8T-query.scw PDB file : Tito_Scwrl_5X8T.pdb: