Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEFTN---------------SDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIA-MAEMGFQGIDINMGCPVPNVAERGKGSGLILRPEVAAQLIEA-AKAGGLPVSVKTRIGYTEMSEMEEWISHLLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGD----IPDRQTGEELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSITGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ 4WFS Chain:A ((8-273)) -------------LILAPMVRVGTLPMRLLALDYGADIVYCEELIDLKMIQCKRVVNEVLSTVDFVAPDDRVVFRT----CEREQNRVVFQMGTS-DAERALAVARLVENDVAGIDVNMG-------------ALLSDPDKIEKILSTLVKGTRRPVTCKIRI-LPSLEDTLSLVKRIERTGIAAIAVHGRKREERPQHPVSCEVIK---AIADTLS--IPVIANGGSHDHIQQYSDIEDFRQATAASSVMVARAAMWNPSIFLKEGLRPLEEVMQKYIRYAVQYDNHYT----------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4WFS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1292 6262 4.85 26.99 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 4.85 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.431

(partial model without unconserved sides chains): PDB file : Tito_4WFS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4WFS-query.scw PDB file : Tito_Scwrl_4WFS.pdb: