Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTARRDLIFHNGAVCVLAVTDEQ-KLILVKQYRKAIEAVSYEIPAGKLE-VGENTAPVAAALRELEEETAYT-GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQSGHICDAKTIMAVQYWELQKK 5I8U Chain:A ((5-180)) ---DFETISSETLHTGAIFALRRDQVRMPGGG-IVTREVVEHFGAVAIVAMD-DNGNIPMVYQYRHTYGRRLWELPAGLLDVAGE--PPHLTAARELREEVGLQASTWQVLVDLDTAPGFSDESVRVYLATGLREVGR-----EQADMTMGWYPIAEAARRVLRGEIVNSIAIAGVLAVHAVT-

General information: TITO was launched using: RESULT: Template: 5I8U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 752 -39258 -52.20 -233.68 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -52.20 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.353

(partial model without unconserved sides chains): PDB file : Tito_5I8U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5I8U-query.scw PDB file : Tito_Scwrl_5I8U.pdb: