Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRG----------LLIAALVFSLLSRWLPYRIGQLLFIVFFGWMIFRF---LSKKIYPRLNENQRYLKYLDRVKEKYHQIK-NKKQVKMDRKSYTFFECPNC-------------HQKQRAPKGKGRIRVTCKT-CGIKFETNV---------------------------------- 5TCX Chain:A ((6-232)) --STKSIKYLLFVFNFVFWLAGGVILGVALWLRHPNTFYVGIYILIAVGAVMMFVGFLGYGAIQQLLGTFFTCLVILFACEVAAGIWGFVNKDQIAKDVKQFYDQALQQAVVDDDANNAKAVVKTFHETLDCCGSSTLTALTTSVLKNNLCPSGSNIISNLFKEDCHQKIDDLFSGKLYLIGIAAIVVAVIMIFEMILSMVLSSGIRN

General information: TITO was launched using: RESULT: Template: 5TCX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 366 -25109 -68.60 -174.37 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : -68.60 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.117

(partial model without unconserved sides chains): PDB file : Tito_5TCX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5TCX-query.scw PDB file : Tito_Scwrl_5TCX.pdb: