Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTQTSLSQSPNDTTSSQDVIEIKTVTRSVGRKATITKEELFQAALNLIGPQKSIASLSLREVAREAGIAPNSFYRHFKDIDELAISLIDRAGIVLRKIIRQARLRASLQDSIIRSSVEIFLQQLDADEGNLSLFLREGFTGSASYKAAVDRQLNFFQQELQEDLIRLERLNNNHLYHPELVAKAITQLVFHMGAKVIDLPIDQRAEVAEQTMIMIRMILEGARHLDEAKIR 3LWJ Chain:A ((9-200)) -------------------------------QNKERRQKILTCSLDLFI-EKGYYNTSIRDIIALSEVGTGTFYNYFVDKEDILKNLLEDFAKQIISSISEYYLVEKDLYERFIETKRLTMEVFAQNETLSEIYSRVAG-SSAPIDQCLKQFEDRLLEFYSRNIEYGIKKGVFKNVPVSPIAHSILAIEKFSLYKWVVLKAITKEEMIEMVLSFHKTLAVGLLVV------

General information: TITO was launched using: RESULT: Template: 3LWJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 669 -89798 -134.23 -467.70 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -134.23 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.599

(partial model without unconserved sides chains): PDB file : Tito_3LWJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3LWJ-query.scw PDB file : Tito_Scwrl_3LWJ.pdb: