Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSNLTLSQFLQQE--KGNLTPELAQVIDTIAATCKTIDQALQKGALAGILGSAGNENVQGETQKKLDVISNDYLIDALKVHPHVGGLASEELDD--FTPAQENGEYLVLFDPLDGSSNIDINMCVGTIFSILPAKNAVTQA--QDFMQAGTQQVAAGYVLYGPSTMMALTVGNGVAFFTLDPETQTFLLTTENVQVSADTQEFAINASNQRHWEQPVKQYIEELLAGKTSVREKDFNMRWVACMVGDVHRILCRGGIFLYPYDLKDPKKAGRLRLMYEANPMSMLIEQAGGASTTGRVRILEIEPTELHQRVPVIIGSKNEVERVTSYH 4GWS Chain:B ((11-324)) ---VTLTRFVMEEGRKARGTGEMTQLLNSLCTAVKAIHRAVRKAGIAHLYGIAGSTNVTGDQVKKLDVLSNDLVINVLKSSFATCVLVSEEDKNAIIVEPEKRGKYVVCFDPLDGSSNIDCLVSIGTIFGIY-RKNSTDEPSEKDALQPGRNLVAAGYALYGSATMLVLAMVNGVNCFMYDPAIGEFILVDRDVKIKKKGSIYSINEGYAKEFDPAITEYIQRKKFPPDN--SAPYGARYVGSMVADVHRTLVYGGIFMYPANKKSPK--GKLRLLYECNPMAYVMEKAGGLATTGKEAVLDIVPTDIHQRAPIILGSPEDV-------

General information: TITO was launched using: RESULT: Template: 4GWS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1673 37914 22.66 123.10 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain B : 0.84 3D Compatibility (PKB) : 22.66 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.494

(partial model without unconserved sides chains): PDB file : Tito_4GWS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4GWS-query.scw PDB file : Tito_Scwrl_4GWS.pdb: