Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLEAGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKEIYGKDQVKTWRRGYTEVPPLTAENR-FDR----RYDHLDPHLIPYGESLEMTVNRIIPLWQDHLAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILDKKIL 5UM0 Chain:C ((9-234)) MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKENGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGRLQGLDKKQTAEKYGDEQVRIWRRSYDTLPPLLDKDDAFSAHKDRRYAHLPADVVPDGENLKVTLERVLPFWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDEDIMGLEIPTGQPLVYKLDDNLKVIEKFY-

General information: TITO was launched using: RESULT: Template: 5UM0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1146 6853 5.98 31.01 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain C : 0.86 3D Compatibility (PKB) : 5.98 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.560

(partial model without unconserved sides chains): PDB file : Tito_5UM0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5UM0-query.scw PDB file : Tito_Scwrl_5UM0.pdb: