Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYDVIVIGAGPSGMTAALYASRSNLSVLMIEQGAPGGQMNNTAEIENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVIC-EDKSYEGKTVIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLTQFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVKTGNEFDLAADGVFIYVGLDPLTAPFRE-AGLTNEAGWIPTDNEMKTNMPGVFAIGDVREKDLRQITTAVGDGGIAGQQVYKYLESLEETTA 4GCM Chain:B ((6-310)) DFDIAIIGAGPAGMTAAVYASRANLKTVMIERGIPGGQMANTEEVENFPGFEMITGPDLSTKMFEHAKKFGAVYQYGDIKSVEDKG-EYKVINFGNKELTAKAVIIATGAEYKKIGVPGEQELGGRGVSYCAVCDGAFFKNKRLFVIGGGDSAVEEGTFLTKFADKVTIVHRRDELRAQRILQDRAFKNDKIDFIWSHTLKSINEKDGKVGSVTLTSTKDGSEETHEADGVFIYIGMKPLTAPFKDLGITN-DVGYIVTKDDMTTSVPGIFAAGDVRDKGLRQIVTATGDGSIAAQSAAEYIEHLND---

General information: TITO was launched using: RESULT: Template: 4GCM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1807 -56251 -31.13 -185.65 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.87 3D Compatibility (PKB) : -31.13 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.544

(partial model without unconserved sides chains): PDB file : Tito_4GCM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4GCM-query.scw PDB file : Tito_Scwrl_4GCM.pdb: