Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTARRDLIFHNG-AVCVLAVTDEQ-KLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT-GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQSGHICDAKTIMAVQYWELQKK 2YVP Chain:A ((2-176)) --SPWERILLEEILSEPV-RLVKERVRTHTGR-ELTYVYRPGPVAASFVLPVT-ERGTALLVRQYRHPTGKFLLEVPAGKVDEGE--TPEAAARRELREEVGAEAETLIPLPSFHPQPSFTAVVFHPFLALKARVVT-PPTLEEGELLESLELPLTEVYALLAKGEIQDASTALTLFYAEPHL-

General information: TITO was launched using: RESULT: Template: 2YVP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 778 -9329 -11.99 -54.24 target 2D structure prediction score : 0.38 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -11.99 2D Compatibility (Sec. Struct. Predict.) : 0.38 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.340

(partial model without unconserved sides chains): PDB file : Tito_2YVP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YVP-query.scw PDB file : Tito_Scwrl_2YVP.pdb: