Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSEEMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNGLGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGKFIDVYLMGKLIG 2GE3 Chain:C ((6-169)) --DTVTIKPIRAEHVESFHRALDAVSRERKYLSFLE---APPLEAVRAFVLDMIE-NDHPQFVAIADGDVIGWCDIRRQDRATRAHCGTLGMGILPAYRNKGLGARLMRRTLDAAHEF-GLHRIELSVHADNARAIALYEKIGFAHEGRARDAVSID-GHYIDSLNMAIIFG

General information: TITO was launched using: RESULT: Template: 2GE3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 726 -115871 -159.60 -706.53 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain C : 0.76 3D Compatibility (PKB) : -159.60 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.585

(partial model without unconserved sides chains): PDB file : Tito_2GE3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GE3-query.scw PDB file : Tito_Scwrl_2GE3.pdb: