Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQDTPLIIGSRSFQSRLLVGTGKYKDLNETDLAIQASGAEIVTVAIRRVNIGQNPDQPNLLSVIPPEKYTILPNTAGCFDADSAVRTCMLARALLDGHNLVKLEVLGDEKTLY---PNVTETLKAARTLIDDGFEIMVYTSDDPIIAQELESMGCVAIMPLGSLIGSGLGILNPHTISIIKENA--KVPVLVDAGVGTASDAAIAMELGCDGVLMNTAIAAAQNPILMASAMKKAVEAGREAFLAGRMPRKRMANASSPETGYFFK 2HTM Chain:C ((1-241)) --MDTWKVGPVELKSRLILGSGKYEDFGVMREAIAAAKAEVVTVSVRRVELKA-PGHVGLLEALE--GVRLLPNTAGARTAEEAVRLARLGRLLT-GERWVKLEVIPDPTYLLPDPLETLKAAERLI---EEDFLVLPYMGPDLVLAKRLAALGTATVMPLAAPIGSGWGVRTRALLELFAREKASLPPVVVDAGLGLPSHAAEVMELGLDAVLVNTAIAEAQDPPAMAEAFRLAVEAGRKAYLAGPMRP----------------

General information: TITO was launched using: RESULT: Template: 2HTM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1350 -62905 -46.60 -266.54 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain C : 0.80 3D Compatibility (PKB) : -46.60 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.590

(partial model without unconserved sides chains): PDB file : Tito_2HTM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HTM-query.scw PDB file : Tito_Scwrl_2HTM.pdb: