Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGRKTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAVYQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQRNENPVKSGTGELTI 5ISQ Chain:X ((2-151)) -LSILVAHDLQRVIGFENQLPWHLPNDLKNVKKLSTGHTLVMGRKTFESIG-KPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLP----GHVFIFGGQTLYEEMIDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTF-------------------

General information: TITO was launched using: RESULT: Template: 5ISQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain X - contact count / total energy / energy per contact / energy per residue : 689 -109421 -158.81 -729.47 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain X : 0.84 3D Compatibility (PKB) : -158.81 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.509

(partial model without unconserved sides chains): PDB file : Tito_5ISQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ISQ-query.scw PDB file : Tito_Scwrl_5ISQ.pdb: