Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRFYSLYLHDDACKNRQNLSSCRTYSVSLEFESRRDNMSNENQKPTSPTEQAQSSDKVSPFLSSPLPQGTPQGQQQSLQQTLTDTPVSGSVPKYNLPRGAGNTGNVGETTHFGYSTVRTEDKAQKVAEVFHSVASKYDLMNDLMSFGIHRLWKRFAINMSGVRRGQHVLDIAGGTGDLAKVFSREVGPQGHVVLSDINESMLNVGRDRLIDAGCTNVDFVLANAETLEPFADNSFDLVTISFGLRNVTDKDAALASMFRVLKPGGRLLVLEFSKPVFEPFSKLYDLYSFTALPIMGKLVANDSESYKYLAESIRMHPDQRTLKGMMENAGFQNCDYHNLTGGIVAVHRGFKL 3DH0 Chain:B ((35-182)) -----------------------------------------------------------------------------------------------------------------------------------------------------------------LKEGMTVLDVGTGAGFYLPYLSKMVGEKGKVYAIDVQEEMVNYAWEKVNKLGLKNVEVLKSEENKI-PLPDNTVDFIFMAFTFHELSEPLKFLEELKRVAKPFAYLAIIDWKKEERDK------------------------------GPPPEEVYSEWEVGLILEDAGIRVGRVVEVG------------

General information: TITO was launched using: RESULT: Template: 3DH0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 695 -83254 -119.79 -562.53 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain B : 0.63 3D Compatibility (PKB) : -119.79 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.514

(partial model without unconserved sides chains): PDB file : Tito_3DH0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DH0-query.scw PDB file : Tito_Scwrl_3DH0.pdb: