Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSEVLDVVIVGGGLVGGLTALLLAQGGVQPTVLDAAPVLDVEKTLSVMNPRVLALSQATIHLLKTVNVWDDL-ARQMPYTGMQVWNLNGYGEINFGHESVQRPISEQALGSMVEPSVLNVAIQQKMLEQLTDYRTQVRVTCIEQGVGCWHIQLADGTALKTKLLIGADGANSFVREQAFIDLDVLDYKQAAISCAIKTSKPHHY---VARQIFLPTGPLAYLPMASLEESENGYWQSIVWTLPDDYADEYSALSDREFMQLLTRESQHMLGEVLDVRSRAQFPLKARAAKQYVKAGLALIGDAAHVIHPLAGQGVNIGCLDAAVLCDALLHDLGRGVWAHEQTLMRYEHRRKGQNDAMMHSMSAIGWLESSELFPLIWARNVGLKQVEQISFLKERFMQQANGLGALQNTQYSR 5KOW Chain:A ((3-375)) --HMIDVIIAGGGPTGLMLAGELRLHGVRTVVLEKEPTP-------NQHSRSRGLHARSIEVMDQRGLLERFLAHGEQFRVGGFFAGLA---AEWPA----DLDTAHSYVLAIPQVVTERLLTEHATELGAEIRRGCEVAGLDQDADGVTAELADGTRLRARYLVGCDGGRSTVRRLLGVDFPGEPTRVETLLADVRIDVPVETLTAVVAEVRKTQLRFGAVPAGD-------GFFRLIVPAQGLSADR-AAPTLDELKRCLHATAGTDF-GVHSPRWLSRFGDATRLAERYRTGRVLLAGDAAHIHPPTGGQGLNLGIQDAFNLGWKLAAAIGGW--APPDLLDSYHDERHPVAAEVLDNTRAQMTLLSLDP-GPRAVRRL-MAELVEFPDVNRHLIEKIT------------

General information: TITO was launched using: RESULT: Template: 5KOW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1995 -52094 -26.11 -141.18 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -26.11 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.373

(partial model without unconserved sides chains): PDB file : Tito_5KOW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5KOW-query.scw PDB file : Tito_Scwrl_5KOW.pdb: