Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKQILILAGDGIGPEIVGAAEKVLNKVNEKFNLSLTWEHGLLGGAAID---AHGEPYPAVTSEQAKKADAILLGAVGGPKWDTIERSIRPERGLLKIRSELNLFANLRPAILYPQLADASSLKPEIVAGLDILIVRELTGGIYFGQPRGIRELENGEKQ-GYNTDVYSESEIKRIAKVAFELAGLRGGK-VCSVDKANVLEVTELWKQTVTDLQQANYPNIQLSHMYVDNAAMQLVRAPKQFDVIVTGNLFGDILSDEAAMLTGSIGMLPSASLD--ENGKGMYEPCHGSAPDIAGQNVANPLATILSVAMMLRY----TFREEAAAKAIEDAVGQVLDQGLRTADIMSEGMTKVGTAEMGEAVVAALA 3FMX Chain:X ((5-361)) -SFRIAAIPGDGIGLEVLPEGIRVLEAAALKHGLAL--EFDTFEWASCDYYLQHGKMMPDDWAEQLKQYDAIYFGAVGWP--DKVPDHISLWGSLLKFRREFDQYVNIRPVRLFPGVP--CALANRKVGDIDFVVVRENTEGEY--SSLGGIMFENTENEIVIQESIFTRRGVDRILKYAFDLAEKRERKHVTSATKSNGMAISMPYWDKRTEAMAAHYPHVSWDKQHIDILCARFVLQPERFDVVVASNLFGDILSDLGPACAGTIGIAPSANLNPERNFPSLFEPVHGSAPDIFGKNIANPIAMIWSGALMLEFLGQGDERYQRAHDDMLNAIERVIADGSVTPDM---GGT-LSTQQVGAAISDTLA

General information: TITO was launched using: RESULT: Template: 3FMX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain X - contact count / total energy / energy per contact / energy per residue : 1916 15383 8.03 44.46 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain X : 0.78 3D Compatibility (PKB) : 8.03 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.560

(partial model without unconserved sides chains): PDB file : Tito_3FMX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FMX-query.scw PDB file : Tito_Scwrl_3FMX.pdb: