Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRIILLGPPGAGKGTQAQLICKRYNIPQISTGDMLRAAIREGTELGLKAKSVMESGGLVSDELIIGL----VKERIAQPDCVNGCIFDGFPRTIPQAEALEK-EGISIDHVIEIDVP-DEEIVKRL--SGRRQHPASGRVYHVVYNPPKVEGKDDETGEDLVQRPDDQEETIRKRLASYHTETEQLVGFYQGRAASGENAPTYDKLDGLRTIEDVQKDLFNILDK 1AKE Chain:A ((1-214)) MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSELGKQAKDIMDAGKLVTDELVIALVKERIA-QEDCR---NGFLLDGFPRTIPQADAMKEA-GINVDYVLEFDV-PDELIVDRIVG--RRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQEETVRKRLVEYHQMTAPLIGYYSKEAEAGN--TKYAKVDGTKPVAEVRADLEKILG-

General information: TITO was launched using: RESULT: Template: 1AKE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 969 10144 10.47 49.24 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.87 3D Compatibility (PKB) : 10.47 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.551

(partial model without unconserved sides chains): PDB file : Tito_1AKE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1AKE-query.scw PDB file : Tito_Scwrl_1AKE.pdb: