Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSARHSRLIILGSGPAGYSAAVYAARANLKPTLIAGLQLGGQLTTTTEVDNWPGDPEGLTGPVLMDRMQAHAERFGTELVYDHINEVDLNVRPFVLKG-DMDEYTCDALIIATGATAQYLGLESEQKFMGQGVSACATCDGFFYKNQNVMVVGGGNTAVEEALYLSNIAEHVTLVHRRDSLRSEKILQDHLFAKEKESKISIVWNHEVEEVLGDN---TGVTGVRLKSTKDDSKQEVQVQGLFIAIGHKPNTSMFEGQLNLR-DGYIQVQSGTSGNATATSVAGVFAAGDVADSIYRQAITSAGSGCMAALDAEKYLDNL 4JNQ Chain:A ((23-335)) -TQRHAPVIVIGSGPAGYTAAIYAARAMLKPVVIAGLQQGGQLMITTDVENYPGYAEPVQGPWMMEQMARQAENVGAQIVHDIITEVETTVRPFRLKGDSGTIYTCDALIIATGAQAKWLGLESEQTFMGGGVSACATCDGFFYRGKDVVVVGGGNTAVEEALYLSHIAKSVTIVHRRDGFRAEK---IMQDRLLSRENVSVVWNSVIDEILGTEARGATVTGVRLKNIVTGETQERATHGVFIAIGHAPAVSLFEGKLKQKPNGYLWTAPD----STATDVPGIFAAGDVTDDIYRQAVTAAGMGCMAALEAERWLA--

General information: TITO was launched using: RESULT: Template: 4JNQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1850 -80675 -43.61 -264.51 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.86 3D Compatibility (PKB) : -43.61 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.564

(partial model without unconserved sides chains): PDB file : Tito_4JNQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4JNQ-query.scw PDB file : Tito_Scwrl_4JNQ.pdb: