Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPALFL---QELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYFHETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVSNMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPENIAEYMAKENVDGALVGGASLQADSFLSLLDAVK 4Z0S Chain:A ((3-246)) -RKYFVAANWKCNGTLESIKSLTNSFNNLDFDPSKLDVVVFPVSVHYDHTRKLLQSKFSTGI----QNVSKFGNGSYTGEVSAEIAKDLNIEYVIIGHAERRKYFHETDEDVREKLQASLKNNLKAVVCFGESLEQREQNKTIEVITKQVKA-FVDL-IDNFDNVILVYEPLWAIGTGKTATPEQAQLVHKEIRKIVKDTCGEKQANQIRILYGGSVNTENCSSLIQQEDIDGFLVGNASLK-ESFVDIIKS--

General information: TITO was launched using: RESULT: Template: 4Z0S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1251 -2484 -1.99 -10.31 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -1.99 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.488

(partial model without unconserved sides chains): PDB file : Tito_4Z0S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Z0S-query.scw PDB file : Tito_Scwrl_4Z0S.pdb: