Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAVMISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMAGLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTG---ENPLIGPNEDAMGPRFPDMSQAYTPDYQATAKAAAEKLGLD--LKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEVIVAAHSGLKVLGISCITNLAA-GMQANLSHEEVVETTQRVK--QSFKALVKETLTLL 3FB1 Chain:B ((28-284)) -----------------------EIGIICGSGLGKLADGVKDKITIPYTKIPNFPQT-VVGHSGNLIFGTLSGRKVVVMQGRFHMYEGYSNDTVALPIRVMKLLGVKILMVSNAAGGLNRSLKLGDFVILKDHIYLPGLGLNNILVGPNQEAFGTRFPALSNAYDRDLRKLAVQVAEENGFGNLVHQGVYVMNGGPCYETPAECTMLLNMGCDVVGMSTIPEVVIARHCGIQVFAVSLVTNISVLDVES----------AQRAELMQSWFEKIIEKL---

General information: TITO was launched using: RESULT: Template: 3FB1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1305 -119038 -91.22 -506.54 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.80 3D Compatibility (PKB) : -91.22 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.519

(partial model without unconserved sides chains): PDB file : Tito_3FB1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FB1-query.scw PDB file : Tito_Scwrl_3FB1.pdb: