Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKILVLHTGGTIAMKENMLTGGVSPD-VANPLLDAEITIPKHVELLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG-NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDE-------IHSARYVTKTHTTNVATFRTPTLGPIGLVTKN-RILFLQELLET---KRLD-ISAVDGTIPIVKAYAGMQGDLLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEP-VYDYEGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN 1ZQ1 Chain:A ((91-421)) LPEVTIIGTGGTIASRIDYETGAVYPAFTAEELAKALPEIFEVANVKPKLLFNIFSEDMKPKHWVKIAHEVAKALNSGDYGVVVAHGTDTMGYTAAALSFMLRNLGKPVVLVGAQRSSDRPSSDAAMNLICSVRMATSE---VAEVMVVMHGETGDTYCLAHRGTKVRKMHTSRRDAFRSINDVPIAKIWPNGEIEFLRKDYRKRSDEEVEVDDKIEEKVALVKVYPGISSEIIDFLVDKGYKGIVIEGTGLGHTPNDIIPSIERAVEEGVAVCMTSQCIYGRVNLNVYST---GRKLLKAGVIPCEDMLPETAYVKLMWVLGHTQNLEEVRKMMLT

General information: TITO was launched using: RESULT: Template: 1ZQ1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1909 -64678 -33.88 -204.68 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -33.88 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.527

(partial model without unconserved sides chains): PDB file : Tito_1ZQ1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZQ1-query.scw PDB file : Tito_Scwrl_1ZQ1.pdb: