Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPKEHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAA--GMNIISNCEEVAGQTVFHTHVHLVPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA 3N1T Chain:E ((4-105)) --ETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIPNILIPTVNDVSAEH-EQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIAMHLL---------------------------------

General information: TITO was launched using: RESULT: Template: 3N1T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 380 -68339 -179.84 -683.39 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain E : 0.73 3D Compatibility (PKB) : -179.84 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.508

(partial model without unconserved sides chains): PDB file : Tito_3N1T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3N1T-query.scw PDB file : Tito_Scwrl_3N1T.pdb: