Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLDHHTDGILLAGTTAESPTLTHDEELELFAAVQKIVNGRVPLIAGVGTNDTRDSIEFVKEVAEFGGFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVELTPETMLRLAD-HPNIIGVKECTSLANMAYLIEHK---PEEFLVYTGEDGDAFHAMNLGADGVISVASHTNGDEMHEMFIAIAESDVKKAAAIQRKFIPKVNALFSYPSPAPVKAVLNYMGFEAGPTRLPLVPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY 4I7U Chain:D ((20-290)) -----------IPALITPFTDNGAVDEQAFAAHVEWQIAEGSNGLVPVGTTGESPTLSHDEHKRVVELCIEVAAKRVPVIAGAGSNNTDEAIELALH-AQDAGADALLVVTPYYNKPTQKGLFAHFSAVAEAVKLPIVIYNIPPRSVVDMSPETMGALVKAHKNIVGVKDAT--GKLDRVSEQRISCGKDFIQLSGEDSTALGFNAHGGVGCISVSANVAPRLCSEFQAAMLAGDYAKALEYQDRLMPLHRAIFMEPGVCGTKYALSKTRGCNRKVRSPLMSTLE------------------------------

General information: TITO was launched using: RESULT: Template: 4I7U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1514 -118296 -78.13 -443.05 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain D : 0.77 3D Compatibility (PKB) : -78.13 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.550

(partial model without unconserved sides chains): PDB file : Tito_4I7U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4I7U-query.scw PDB file : Tito_Scwrl_4I7U.pdb: