Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLYFMYRIIERRLSMKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYW--FKTHPEDFFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFYNAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKVHYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLELDGKIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVLWSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEE--YQTPWMPDQYFKEYEWPERRMNPYRGWEIFPKAIYDIAMIVKEEYGNIPWFISENGMGVENEARFIDENGVIDDVYRIEFYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISV--DLETQK---RTIKSSGRWYRKVSDNNGFEVEIEE 4ZE5 Chain:B ((16-485)) --------------------FPPEFLWGAASAAYQVEGAWNEDGKG-LSVWDVFAKQPGRTFKGTNGDVAVDHYHRYQEDVALMAEMGLKAYRFSVSWSRVFPD-GNGAVNEKGLDFYDRLIEELRNHGIEPIVTLYHWDVPQALMDAYGAWESRRIIDDFDRYAVTLFQRFGDRVKYWVTLNQQNIFISFGYRLGLHPPGVKDM-KRMYEANHIANLANAKVIQSFRHYVPDGKIGPSFAYSPMYPYDSRPENVLAFENAEEFQNHWWMDVYAWGMYPQAAWNYLESQGLEPTVAPGDWELLQAAKPDFMGVNYYQTTTV--EHNPPDGV---SSGIPGLFKTVRNPHVDTTNW-DWAIDPVGLRIGLRRIANRY-QLPILITENGLG---EFDTLEPGDIVNDDYRIDYLRRHVQEIQRAITDGVDVLGYCAWSFTDLLSWLNGYQKRYGFVYVNRDDESEKDLRRIKKKSFYWYQRVIETNGAEL----

General information: TITO was launched using: RESULT: Template: 4ZE5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2650 32524 12.27 72.92 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : 12.27 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.490

(partial model without unconserved sides chains): PDB file : Tito_4ZE5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZE5-query.scw PDB file : Tito_Scwrl_4ZE5.pdb: