Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSEVLDVVIVGGGLVGGLTALLLAQGGVQPTVLDAAPVLDVEKTLSVMNPRVLALSQATIHLLKTVNVWDDL-ARQMPYTGMQVWNLNGYGEINFGHESVQRPISEQALGSMVEPSVLNVAIQQKMLEQLTDYRTQVRVTCIEQGVGCWHIQLAD---GTALKTKLLIGADGANSFVREQAFIDLDVLDYKQAAISCAIKTSKPHHYVARQIFLPTGPLAYLPMASLEESENGYWQSIVWTLPDDYADEYSALSDREFMQLLTRESQHMLGEVLDVRSRAQFPLKARAAKQYVKAGLALIGDAAHVIHPLAGQGVNIGCLDAAVLCDALLHDLGRGVWAHEQTLMRYEHRRKGQNDAMMHSMSAIGWLESSELFPLIWARNVGLKQVEQISFLKERFMQQANGLGALQNTQYSR 2QA1 Chain:A ((8-349)) HRSDAAVIVVGAGPAGMMLAGELRLAGVEVVVLERLVE-T-------GESRGLGFTARTMEVFDQRGILPRFGEVETSTQ-GH-FG--GL-PIDFGV-----LEGAWQAAKTVPQSVTETHLEQWATGLGADIRRGHEVLSLTDDGAGVTVEVRGPEGKHTLRAAYLVGCDGGRSSVRKAAGFDFPGTAATMEMYLADIKGVELQ-PRMIGETLPGGMVMVGPLPG-------GITRIIVCERGTPP----PPSWHEVADAWKRLTGDDI-AHAEPVWVSAFGNATRQVTEYRRGRVILAGDSAHIHLPAGGQGMNTSIQDAVNLGWKLGAVVNGT--ATEELLDSYHSERHAVGKRLLMNTQAQGLLF---------------------------------------------

General information: TITO was launched using: RESULT: Template: 2QA1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1901 -32553 -17.12 -98.05 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -17.12 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.410

(partial model without unconserved sides chains): PDB file : Tito_2QA1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2QA1-query.scw PDB file : Tito_Scwrl_2QA1.pdb: