Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAWQNVEVVHVVAMDKNHCIGKGNALPWHISADLKHFKEITQGGVVIMGRKTLESMGRALPNRVNWVITRDINWHFDGVKIAYSIEDALNAALEDAKNTEKQALFIIGGGEIFKQTLSIADRLELTHVDLDVQGDAHYPTIPSEFH----KTASEQQVDEKSGTSFEFATYKK 2W9S Chain:B ((6-152)) ----------IVAHDKQRVIGYQNQLPWHLPNDLKHIKQLTTGNTLVMARKTFESIGKPLPNRRNVVLTNQASFHHEGVDVINSLDEIKELS---------GHVFIFGGQTLYEAMIDQVDDMYITVIDGKFQGDTFFP--PYTFEDWEVESSVEGQLDEKNTIPHTF-----

General information: TITO was launched using: RESULT: Template: 2W9S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 593 -79396 -133.89 -555.22 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : -133.89 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.501

(partial model without unconserved sides chains): PDB file : Tito_2W9S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2W9S-query.scw PDB file : Tito_Scwrl_2W9S.pdb: