Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQFTEEQLLIRDMAKSFAQEQIKPNASDWDRDGTFPKETLTQMGQLGFMGMLVSEEWGGSDTGNLAYVLALEEVAAADGATSTIMSVHNSVGCVPILKFGTDEQKERFLKPLAQGEMIGAFALTEPHTGSDAAAIKTRAVKDGDDYILNGAKQFITSGNNAGVIIVFAVTDPSAGKKGISAFLVPRETPGYEVIRVEEKLGLHASDTCQIALTDVRIHKSLMLGKEGEGLKIALANLEGGRIGIAAQAVGLARAALEEATRYAKERITFGKPIFEHQTIAFRLASMATEIEAARQLVHYAARFKEVGQPCLNEASMAKLFASEMTERVCSSALQVFGGYGYLRDFPIERIYRDARICQIYEGTSDIQRLVIARSL 2JIF Chain:D ((28-400)) -TFTDEEMMIKSSVKKFAQEQIAPLVSTMDENSKMEKSVIQGLFQQGLMGIEVDPEYGGTGASFLSTVLVIEELAKVDASVAVFCEIQNTLINTLIRKHGTEEQKATYLPQLTT-EKVGSFCLSEAGAGSDSFALKTRADKEGDYYVLNGSKMWISSAEHAGLFLVMANVDPTIGYKGITSFLVDRDTPGLHIGKPENKLGLRASSTCPLTFENVKVPEANILGQIGHGYKYAIGSLNEGRIGIAAQMLGLAQGCFDYTIPYIKERIQFGKRLFDFQGLQHQVAHVATQLEAARLLTYNAARLLEAGKPFIKEASMAKYYASEIAGQTTSKCIEWMGGVGYTKDYPVEKYFRDAKIGTIYEGASNIQLNTIAKHI

General information: TITO was launched using: RESULT: Template: 2JIF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 2166 -188283 -86.93 -504.78 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain D : 0.85 3D Compatibility (PKB) : -86.93 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.520

(partial model without unconserved sides chains): PDB file : Tito_2JIF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2JIF-query.scw PDB file : Tito_Scwrl_2JIF.pdb: