Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPPFVLVDGSYFLFRAYHALPPLTTSTGLHTNAIRGAISAIQKLMRRMQPTHMAVIFDTPEPTFRHKLSPIYKGDRPTMPEELSQQIPYLHALIKAQGIPLYSLPGAEADDIIGTLAKRAVKEGHHVLISTGDKDMAQLVNDYVKLEDSFKDRVLGTDGVFEKFGVWPNQIIDYLTLMGDASDGIMGVPGVGAKTAAKLLTEYGTLDNIIANVDQLKGKLSQNIKDNLDNIKLDHQLASIVCDLPLELDWHELKLTDPNVDALRNLYTELEFRNQLQSLDHPNNPNSSSYKQQASQTIVQNEVKPAQTIETEDQASLTSQDDQLGTANYHTVLTQEAWDQLWQRMQNADHFAIDTETTSLDYRIAEMVGFSIAFDAKDAYYVPLAHNYENAPQQLDREQVLAQIKPVLENEAVKKIGHHL-KYDAHIFANHGIE-----LKGWYFDSMLASYVLNAAATRHGMDDVARVYLSHLTTTFEQIAGKGAKQ--KTFNQIEIEVAAHYAAEDAHVTYRLYEVLSNKLK-AHPELENILYNIEMPVARILSGMEEDGIRLDHAFLDKLSVEFAKTMEDLENQAMEIAGETFNIASPKQVGEILFDKLGIKGGKKTATGQYSTSESVLEKI--EHPLTEIILEHRGLAKLKSTYTDR-LIEQSHNDTHRVHTSYHQALTATGRLSSSDPNLQNIPIRRQIGRQIRKAFIAPE-GRVLLAADYSQIELRLMAHFSQDD-ALVHAFQHGQDVHRRTAAEVLGIAIEDVTNDQRRQAKAVNFGLLYGMSEFGLIRQLGFTREESQNYIKQYFQRYPGIYEYMQRTRQVA-SEQGFVETILGRRLYTPDIDARNVMVRKAAERAAINAPLQGSAADIIKLAMIEVDKILPKD--QAKLLLQVHDELVFEV-DSNIADELSKQIADVMQSVLE-ISVPLVVEVGQGNNWDDAH 2KFZ Chain:A ((1-605)) ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MISYDNYVTILDEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDAPDQISRERALELLKPLLEDEKALKVGQNLK-YDRGILANY---GIELR--GIAFDTMLESYILNSVAGRHDMDSLAERWLKHKTITFEEIAGKGK--NQLTFNQIALEEAGRYAAEDADVTLQLHLKMWPDLQK-HKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAELEKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKT----PSTSEEVLEELALDYPLPKVILEYRGLAKLKSTYTD-KLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVRNEEGRRIRQAFIAP-EDYVIVSADYSQIELRIMAHLSRD-KGLLTAFAEGKDIHRATAAEVFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGVLEYMERTRAQAK-EQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADIIKRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVH-KDDVDAVAKQIHQLMENCT-RLDVPLLVEVGSGENWDQAH

General information: TITO was launched using: RESULT: Template: 2KFZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2848 8460 2.97 14.54 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : 2.97 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.523

(partial model without unconserved sides chains): PDB file : Tito_2KFZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2KFZ-query.scw PDB file : Tito_Scwrl_2KFZ.pdb: