Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPALFL---QELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYFHETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVSNMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPENIAEYMAKENVDGALVGGASLQADSFLSLLDAVK 5EYW Chain:B ((5-247)) -RKFFVGGNWKMNGDKAAIDGIISFMKTG-PLSPNTEVVVGCPQCYLMYTREHMPANIG----IAAQNCYKVAKGAFTGEISPAMVKDCGCEWVILGHSERRNVFGEPDQLISEKVGHALEAGLKVIPCIGEKLEDREGNRTQEVVFAQMKALLPNIS--DWSRVVLAYEPVWAIGTGKTASPEQAQEVHADLRQWLRDNVNAEVAESTRIIYGGSVSAGNCQELAKKGDIDGFLVGGAALKPD-FVQIINA--

General information: TITO was launched using: RESULT: Template: 5EYW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1327 -4465 -3.36 -18.60 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.81 3D Compatibility (PKB) : -3.36 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.531

(partial model without unconserved sides chains): PDB file : Tito_5EYW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EYW-query.scw PDB file : Tito_Scwrl_5EYW.pdb: