Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------MIVL-TLGGGLGNQMFQYGYARYIQKIHREKF-IYINDSEVIKEADRFNSLGNLNTVNIKVLPRIISKP-------LNETERLVRKIMV-----------RLFGV-AGFNESAIFQSLNKFGIYYHPSVYKFYESLKTGF----PIK-II--EGGF-QSWKYLETCPEIKQELRVKYEPMG-------ENLRLLNLI-SQSESVCVHIRRGDYLSPKYKHLNVCDYQYYFESMNYIISKLNNPTFFIFSNTSDDLDWIKENYSLPG-KIVYVKNDNPDYEE-----------LRLMYSCKH-----------FIISNSTFSWWAQYLS----NNSGIVIAPEIWNRLNH-DGIADLYMPNWITMKVNR----- 1FUU Chain:B ((11-394)) QIQTNYDKVVYKFDDMELDENLLRGVFGYGFEE-PSAIQQRAIMPIIEGHDVLAQAQ--SGTGKTGTFSIAALQRIDTSVKAPQALMLAPTRELALQIQKVVMALAFHMDIKVHACIGGTSFVEDAEGLRDAQIVVGT-PGRVFDNIQRRRFRTDKIKMFILDEADEMLSSGFKEQIYQIFTLLPPTTQVVLLSATMPNDVLEVTTKFMRNPVRILVKKDELTLEGIKQFYVNVEEEEYKYECLTDLYDSISVTQAVIFCNTRRKVEELTTKLRNDKFTVSAIYSDLPQQERDTIMKEFRSGSSRILISTDLLARGIDVQQVSLVINYDLPANKENYIHRIGRGGKGVAINFVTNEDVGAMRELEKFYSTQIEELPSDIATLLN

General information: TITO was launched using: RESULT: Template: 1FUU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1326 39983 30.15 133.72 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain B : 0.63 3D Compatibility (PKB) : 30.15 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.237

(partial model without unconserved sides chains): PDB file : Tito_1FUU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FUU-query.scw PDB file : Tito_Scwrl_1FUU.pdb: