Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQDTPLIIGSRSFQSRLLVGTGKYKDLNETDLAIQASGAEIVTVAIRRVNIGQNPDQPNLLSVIPPEKYTILPNTAGCFDADSAVRTCMLARALLDGHNLVKLEVLGDEKTLY---PNVTETLKAARTLIDDGFEIMVYTSDDPIIAQELESMGCVAIMPLGSLIGSGLGILNPHTISIIKENAKVPVLVDAGVGTASDAAIAMELGCDGVLMNTAIAAAQNPILMASAMKKAVEAGREAFLAGRMPRKRMANASSPETGYFFK 1TYG Chain:A ((2-242)) -----LTIGGKSFQSRLLLGTGKYPSFDIQKEAVAVSESDILTFAVRRMNIFE-ASQPNFLEQLDLSKYTLLPNTAGASTAEEAVRIARLAKASG-LCDMIKVEVIGCSRSLLPDPVETLKASEQLL---EEGFIVLPYTSDDVVLARKLEELGVHAIMPGASPIGSGQGILNPLNLSFIIEQAKVPVIVDAGIGSPKDAAYAMELGADGVLLNTAVSGADDPVKMARAMKLAVEAGRLSYEAGRIPLKQY-------------

General information: TITO was launched using: RESULT: Template: 1TYG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1342 -75799 -56.48 -318.48 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -56.48 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.551

(partial model without unconserved sides chains): PDB file : Tito_1TYG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TYG-query.scw PDB file : Tito_Scwrl_1TYG.pdb: