Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLNVLKHFKEKQMPSFQSADAQINYQTFGEPSSPALVFSNSLGTNYGMWQKQFNELKDQFFVICYDNRGHGSSSTPDGPYTVEQLGEDVVRLLDHLNISKAAFCGISMGGLTGQWLAIHYPNRFSHVVVANTAAKIGQEQAWLDR--A-KLVRE---QGLQPIA----TTAASRWFTDPFIQ-SHPSIVNNLCNDLSAGSA--MGY--ANC------CEALAK--ADVREQLKDIKIPVLVIAGPQDPVTTVADGEFMQQRIPQSKLAEID-ASHISNVEQPEVFNKILKDFLVN 4K2A Chain:C ((6-287)) -------------HHRAVLGSTMAYRETGRSDAPHVLFLHGNPTSSYIWRNIMPLVAPVGHCIAPDLIGYGQSGKPDISYRFFDQADYLDALIDELGIASAYLVAQDWGTALAFHLAARRPQLVRGLAFMEFIRPMRDWSDFHQHDAARETFRKFRTPGVGEAMILDNNAFVERVLPGSILRTLSEEEMAAYRAPFATRESRMPTLMLPRELPIAGEPADVTQALTAAHAALAASTYPKLLFVGSPGALVSPAFAAEFAKTLKHCAVIQLGAGGHYLQEDHPEAIGRSVAGWIAG

General information: TITO was launched using: RESULT: Template: 4K2A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1331 -80360 -60.38 -311.47 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain C : 0.68 3D Compatibility (PKB) : -60.38 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.507

(partial model without unconserved sides chains): PDB file : Tito_4K2A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4K2A-query.scw PDB file : Tito_Scwrl_4K2A.pdb: