Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPNCYAIHFYLECFMRKSLIWINLIIAMGALGFTNVVNAKDEYPLHMAAANDDIQLIKHILSQKTLIDA-RDETGSTALMVATRANNIHAAHMLIEAGADVNAKDNIQDSPYLYAGAQGYLKILRMTLMHGADLKSTNRYGGTALIPAAERGH-VETVRTLIAAGVNINHVNN-LGWTALLEAIILGNGKSNYQQIVALLLKAGANPNLADKDGITPLQHARTRGYREIEKLLLVAGAK 3EU9 Chain:C ((19-234)) ---------------YGIYERCRELVEAGYD--VRQPDKENVTLLHWAAINNRIDLVKYYISKGAIVDQLGGDLNSTPLHWATRQGHLSMVVQLMKYGADPSLIDGEGCSCIHLAAQFGHTSIVAYLIAKGQDVDMMDQNGMTPLMWAAYRTHSVDPTRLLLTFNVSVNLGDKYHKNTALHWAVLAG-----NTTVISLLLEAGANVDAQNIKGESALDLAKQRKNVWMINHLQEARQ-

General information: TITO was launched using: RESULT: Template: 3EU9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1188 -56190 -47.30 -263.80 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain C : 0.67 3D Compatibility (PKB) : -47.30 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.575

(partial model without unconserved sides chains): PDB file : Tito_3EU9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EU9-query.scw PDB file : Tito_Scwrl_3EU9.pdb: