Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPELPEVETTKTSLFPLL-NQKVLSVEVRNPSLRWPIPDNVQKLVGQRLIGLNRRSKYILAEFEQ-DQMLWHLGMSGSFRLCQPNDELRKHDHLIIQFEDQQLRYHDPRRFGCILWL--NPETQGKLIDTLGPEPLSTDFHAEYLASKLKNKSVGIKIALMDNHVVVGVGNIYATESLFNVGIHPAQPAGDLTMQQIEKLVVEIKRILKSAIDLGGSTLRD--YSNAMGENGYFQQTLLAYGRAREMCVNCETTLENLKLGQRASVFCPQCQPLKKLRKP 1EE8 Chain:A ((1-266)) -PELPEVETTRRRLRPLVLGQTLRQVVHRDP----ARYRNTALAEGRRILEVDRRGKFLLFALEGGVELVAHLGMTGGFRL-----EPTPHTRAALVLEGRTLYFHDPRRFGRLFGVRRGDYREIPLLLRLGPEPLSEAFAFPGFFRGLKESARPLKALLLDQRLAAGVGNIYADEALFRARLSPFRPARSLTEEEARRLYRALREVLAEAVELGGSTLSDQSYRQPDGLPGGFQTRHAVYGREGLPCPACGRPVERRVVAGRGTHFCPTCQG----EGP

General information: TITO was launched using: RESULT: Template: 1EE8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1416 -8382 -5.92 -32.24 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -5.92 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.332

(partial model without unconserved sides chains): PDB file : Tito_1EE8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EE8-query.scw PDB file : Tito_Scwrl_1EE8.pdb: