Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFISFEGTEGVGKTTLIRKIHQHFEEQ----GKQVVLTREPGGTPLAEQIRSMLLAVNHDENMSHDTELLLIYAARAQHLQQVILPALESNKIVLSDRFTDASFAYQCSGRGLSQDKLQLLNQNFV-SRMPEVTFWLDAPIELGMNRARERGALDRFEQEKLSFFTKVREGYETLWKAEPERIKRLDATQSPDQVFEQALQYLA 3V9P Chain:A ((26-224)) KFITFEGID---KTTHLQWFCDRLQERLGPAGRHVVVTREPGGTRLGETLREILLNQ----PMDLETEALLMFAGRREHLALVIEPALARGDWVVSDRFTDATFAYQGGGRGLPRDKLEALERWVQGGFQPDLTVLFDVPPQIASARRGAVRMP---ESESDAFFARTRAEYLRRAQEAPHRFVIVDSSEPIAQIRKQLEGVL-

General information: TITO was launched using: RESULT: Template: 3V9P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 774 10712 13.84 56.98 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : 13.84 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.571

(partial model without unconserved sides chains): PDB file : Tito_3V9P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3V9P-query.scw PDB file : Tito_Scwrl_3V9P.pdb: