Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAAKRTDELIPLCHPLPIYRADVEYELEHDFVKIIAAVETIGPTGVEMEALTAASLAGLTIYDMLKPHCEPEELWMDQCKLLKKKGGKSHFKRVLRQPVSAAVIVLSDTVAA-----GRKPDTAGKSVVETLTEAGFDPIHYQILPDEADDLKELVLELT--KSYACIMTVGGTGIGKRDITVDTLEPLLERKLDGLMEAARSFGQK---RTPYAAMSRGVAGFIDRSLVVALPGSRGGASESMA-AILPALVHIFDVCRDLPHPGGYE 3IWT Chain:A ((13-177)) -----------------------------------------------------------------------------------------------PKSLNFYVITISTSRYEKLLKKEPIVDESGDIIKQLLIENGHKIIGYSLVPDDKIKILKAFTDALSIDEVDVIISTGGTGYSPTDITVETIRKLFDREIEGFSDVFRLVSFNDPEVKAAAYLTKASAGIIGKKIVYLLPGSPDAVKLALKELILPEVGHLVYLVR---------

General information: TITO was launched using: RESULT: Template: 3IWT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 791 -32758 -41.41 -212.71 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -41.41 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.550

(partial model without unconserved sides chains): PDB file : Tito_3IWT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3IWT-query.scw PDB file : Tito_Scwrl_3IWT.pdb: