Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQQQVIIVGGGMVGLSLSLMLAKANIAVKLLEAVKYPNYDDQNVAPYHSSFDARNTALSRRSVQIYQKLGLWDALQQHATPILQVHITEQGSFGKARLIAEQEKVESFGQVIENAWLGRVLLTQVRQQPLIELIDGVQVTALTQDAEQVHIEAQRGDEVLKLESKLLIAADGRDSFCRQAIGVGVDVHDYDQVAIVTTVQTSKPHEHVGFERFSALGPLALLPLPGEYRRSVVWPVKKGTEGEWLGEENDQHFLDALQKTYGDRAGKFEKTGKRFSYPLSQVLAHKQAVGRIILMGNAAHTIHPVAGQGFNLCLRDADVLLRYLVNQLSASDDIGNPDNLLAYEEARLSDQQRVIKFCDTVVRGFSNQNPLLKLIRNTGLIAFDVIPGVKPLVANYAMGLKA 2QA2 Chain:A ((11-351)) SDASVIVVGAGPAGLMLAGELRLGGVDVMVLEQLPQR------------TGESRGLGFTARTMEVFDQRGILPAFGPVETSTQG-HF-GG--RP-VDFGVLE-GAHYGVKAVPQSTTESVLEEWALGR-GAELLRGHTVRALTDEGDHVVVEVEGPDGPRSLTTRYVVGCDGGRSTVRKAAGFDFPGTSASREMFLADIRGCEITP-RPIGETVPLGMVMSAPLGD-GVDRIIVCERGAPARRRTGPPPYQEVAAAWQRLTGQDI-SHGEPVWVSAFGDPARQVSAYRRGRVLLAGDSAHVHLPAGGQGMNVSVQDSVNLGWKLAAVVS-GR--APAGLLDTYHEERHPVGRRLLMNTQAQGMLFL------------------------------------

General information: TITO was launched using: RESULT: Template: 2QA2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1924 -194271 -100.97 -569.71 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -100.97 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.414

(partial model without unconserved sides chains): PDB file : Tito_2QA2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2QA2-query.scw PDB file : Tito_Scwrl_2QA2.pdb: