Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFISFEGTEGVGKTTLIRKIHQHFEEQ----GKQVVLTREPGGTPLAEQIRSMLLAVNHDENMSHDTELLLIYAARAQHLQQVILPALESNKIVLSDRFTDASFAYQCSGRGLSQDKLQLLNQNFV-SRMPEVTFWLDAPIELGMNRARERGALDRFEQEKLSFFTKVREGYETLWKAEPERIKRLDATQSPDQVFEQALQYLA 3V9P Chain:B ((26-224)) KFITFEGID---KTTHLQWFCDRLQERLGPAGRHVVVTREPGGTRLGETLREILLNQ----PMDLETEALLMFAGRREHLALVIEPALARGDWVVSDRFTDATFAYQGGGRGLPRDKLEALERWVQGGFQPDLTVLFDVPPQIASARRGAVRMPDKFESESDAFFARTRAEYLRRAQEAPHRFVIVDSSEPIAQIRKQLEGVL-

General information: TITO was launched using: RESULT: Template: 3V9P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 777 13035 16.78 68.25 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : 16.78 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.550

(partial model without unconserved sides chains): PDB file : Tito_3V9P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3V9P-query.scw PDB file : Tito_Scwrl_3V9P.pdb: