Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MILKIEDLVMSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKVSLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLT-----SGDLVL-EIIGSVRSLLTCERVALNFLQHLSGIASMTAAYVEALGDDCIK--VFDTRKTTPNLRLFEKYAVRVGGGYNHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGADIIMLDNMSLEQIEQAITLIAGRSR---IECSGNIDMTTISRFRGLAIDYVSSGSLTHSAKSLDFSMKGLTYLDV 3C2O Chain:A ((21-291)) -----------------------------LSEDVPSFDFGGYVVGSDLKEA--NLYCKQDGMLCGVPFAQEVFNQCELQVEWL----FKEGSFLEPSKNDSGKIVVAKITGPAKNILLAERTALNILSRSSGIATASHKIISLARSTGYKGTIAGTRKTTPGLRRLEKYSMLVGGCDTHRYDLSSMVMLKDNHIWATGSITNAVKNARAVCGFAVKIEVECLSEDEATEAIEAGADVIMLDN------------------HFLLECSGGLN-------LCDDIDIYSTSSIHQGTPVIDFSLK-------

General information: TITO was launched using: RESULT: Template: 3C2O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1085 -29624 -27.30 -127.69 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -27.30 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.412

(partial model without unconserved sides chains): PDB file : Tito_3C2O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3C2O-query.scw PDB file : Tito_Scwrl_3C2O.pdb: