Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKQILILAGDGIGPEIVGAAEKVLNKVNEKFNLSLTWEHGLLGGAAIDAHGEPYPAVTSEQAK-KADAILLGAVGGPKWDTIERSIRPERGLLKIRSELNLFANLRPAILYPQLADASSLKPEIVAGLDILIVRELTGGIYFGQPRGIRELENGEKQGYNTDVYSESEIKRIAKVAFELAGLR-----GGK--------VCSVDKANVLEVTE-LWKQTVTDLQQ--ANYPNIQLSHMYVDNAAMQLVRAPKQFDVIVTGNLFGDILSDEAAMLTGSIGMLPSASLDENGKGMYEPCHGSAPDIAGQNVANPLATILSVAMMLRYTFREEAAAKAIEDAVGQVLDQG-LRTADIMSEGMTKVGTAEMGEAVVAALA 3TY3 Chain:A ((15-364)) ------LIPADGIGKEVVPAARRLMENLPAKHKLKFDFIDLDAGWGTFERTGKALPERTVERLKTECNAALFGAVQSPTHKVAGYS----SPIVALRKKMGLYANVRPV----KSLDGAKGKP-----VDLVIVRENTECLYVKEERMVQNTP-GKRVAEAIRRISEEASTKIGKMAFEIAKSRQKIRESGTYSIHKKPLVTIIHKSNVMSVTDGLFRESCRHAQSLDPSYASINVDEQIVDSMVYRLFREPECFDVVVAPNLYGDILSDGAASLIGSLGLVPSANVGDNFV-MSEPVHGSAPDIAGRGIANPVATFRSVALMLEFMGHQDAAAD-IYTAVDKVLTEGKVLTPDLGG----KSGTNEITDAVLANI-

General information: TITO was launched using: RESULT: Template: 3TY3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1796 -28689 -15.97 -86.41 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -15.97 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.485

(partial model without unconserved sides chains): PDB file : Tito_3TY3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TY3-query.scw PDB file : Tito_Scwrl_3TY3.pdb: