Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQLISLPKQILQCGQLQLDLSQPHVMGILNVTPDSFSDGGKHNQLDQAVDHALSMIEQGATIIDIGGESTRPGASEVAVEEEVRRVVPVVEALSHH-NVILSIDTSQPEVIRAAKAAGAHIWNDVRALTG-PNALKTAVELDIPVVIMHMRGEP----------------TTMNQLDQYTD--VTLDVMQELQQRIDEALAAGVKKHNIIVDPGFGFAKNAQQNLKLLKEFWKLNELGYPILSGLSRKRFIGEALQGA----------PADQRAVGSVTGHLLSIQQGASIVRAHDVKEMHEAILVWKAMQQA 2VEG Chain:A ((7-302)) -------------------HAKTVICGIINVTP---------FALEQALQQARKLIAEGASMLDIGGE-----SSYVEIEEEIQRVVPVIKAIRKESDVLISIDTWKSQVAEAALAAGADLVNDITGLMGDEKMPHVVAEARAQVVIMFNPVMARPQHPSSLIFPHFGFAFT--ELADFETLPIEELMEAFFERALARAAEAGIAPENILLDPGIGFGLTKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEENGFEVNPETELGFRNRDTASAHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLA

General information: TITO was launched using: RESULT: Template: 2VEG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1264 -29707 -23.50 -119.78 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -23.50 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.497

(partial model without unconserved sides chains): PDB file : Tito_2VEG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2VEG-query.scw PDB file : Tito_Scwrl_2VEG.pdb: