Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTVQMEYEKDVKVAALDG-KKIAVIGYGSQGHAHAQNLRDS------GRDVIIGVRPGKSF-DKAKEDGFDT----Y-TVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGNAVGFAHGFNIHFEFI--KVP-ADVDVFMCAPKGPGHLVRRTYEEG-----FGVPALYAVYQDATGNAKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAGYAPELAYFEVLHEMKL-IVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVK---ENMKAVLADIQNGKFANDFVNDYK-----AGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDDAFKIYN 3FR8 Chain:B ((49-394)) -------------EAFKGIKQIGVIGWGSQGPAQAQNLRDSLAEAKSDIVVKIGLRKGSKSFDEARAAGFTEESGTLGDIWETVSGSDLVLLLISDAAQADNYE-KIFSHMKPNSILGLSHGFLLGHLQSAGLDFPKNISVIAVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSP--FTFATTLEQEYKSDIFGERGILLGAVHGIVEALFRRYTEQGMDEEMAYKNTVEGITGIISKTISKKGMLEVY--NSLTEE-GKKEFNKA-YSASFYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEGLPAFP--MGNIDQTRMWKVGEKVRSTRPENDLGPLHPFTA--

General information: TITO was launched using: RESULT: Template: 3FR8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1401 49591 35.40 156.93 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.71 3D Compatibility (PKB) : 35.40 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.412

(partial model without unconserved sides chains): PDB file : Tito_3FR8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FR8-query.scw PDB file : Tito_Scwrl_3FR8.pdb: